Survival of Mya arenaria and Mya truncata after 12 days in experimental heatwave conditions
Bibliographic record
Abstract
Two species of clams, Mya arenaria (1) and Mya truncata (2), were hand collected from Métis-sur-Mer, Québec, Canada (48° 40' 4.6092" N, 68° 1' 5.9484" W) and by SCUBA diving (~ 10 m) at Godbout, Québec, Canada (49° 19' 25.626" N, 67° 35' 17.034" W) respectively. The clams were brought to the wet labs of the Maurice-Lamontagne Institute in Mont-Joli, Québec for acclimatization to experimental conditions. A clams' ability (1) or inability (0) to bury itself into the substrate (sand) was observed visually to control for it's possible relationship to clam performance variability, as well as their common garden tank (pre.acclim) to control for possible tank variability. After > 1 month in this pre-acclimatization phase, during the months of november and december 2020, clams were measured for morophometrics (length, width, and height) with a vernier caliper to control for the relationship between size and clam physiology, and transferred to the experimental system and subjected to a combination of two stressors: one of seven levels of heatwave (2, 7, 12, 17, 22, 27, or 32 °C) crossed with one of two levels of harvesting (with, without). The true temperature was recorded (truetemp) with HOBO 8K pendant data loggers to measure the variation in real temperature experienced by the clams in each tank. Four tank replicates were used for each of the fourteen (7 x 2) experimental treatments to account for possible tank effects. In each of these tanks (1-4), eight individuals of each species were placed together to increase replication. At the end of the experimental period, mortality was assessed by prodding clams in each tank for each species (mya_spp_heatwave_mortality_data) as a measure of response to the stressors. In surviving individuals, three tissues -- mantle (m), gills (g) and posterior adductor muscle (a) were dissected from each individual to asses inter organ differences and the tissues were flash freezed for metabolomics analysis. A targeted metabolomics analysis was run over the months of March to May 2021 at the Iso-BioKem laboratories in Rimouski, Québec, Canada to quantify 48 metabolites with an Agilent 1260 Infinity II high performance liquid chromatographer (mya_spp_heatwave_metabolomics_data) as a measure of response to the stressors.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".