Guppy gene expression dynamics: effects of sex, reproductive status, feeding, and temperature on reproductive function, methylation, and appetite
Bibliographic record
Abstract
Introduction: This thesis examines the dynamics of gene expression in guppy fish (Poecilia reticulata) under various growth conditions, diets, and temperatures. Understanding the molecular processes underlying methylation, reproduction, and appetite regulation in these fish is the goal of this research. The process by which genetic information is transformed into useful products like proteins is known as gene expression. Guppy reproduction is highly impacted by elements like diet and temperature. This work investigates the effects of these factors on gene expression. Methodology: Male and female guppies (Poecilia reticulata) were housed in 20L aquariums with filtered water at 25°C under a 12-hour light/dark cycle. Fish were acclimated for a week and fed daily with Fancy Guppy meal. For gene expression analysis, fish were divided into experimental groups: 1) fasting vs. feeding for 14 days, 2) temperature effects (25°C, 32°C, and 18°C). Tissues (brain, liver, intestine) were collected post-mortem for RNA extraction using the GeneJET RNA Purification Kit. Total RNA was reverse transcribed using the SensiFAST cDNA Synthesis Kit. Specific gene expression was analyzed via qPCR, and primer design was based on Poecilia reticulata sequences. Data were analyzed using the 2-ΔΔCt method, normalized to reference genes GAPDH, EF-1α, and β-actin. Statistical analyses included ANOVA and non-parametric tests with a significance level of P<0.05. Results: A total of 45 guppies were examined, including 33 females and 12 males, with females classified into three reproductive stages. Analysis of brain gene expression showed no significant differences in orexin (OX) or NPY levels between groups, but Kiss2 expression was higher in females with late-stage embryos, while AVP was elevated in males. Intestinal and liver genes expression showed no significant changes. In fasting experiments, females consumed more food than males, with brain orexin in fast male and hepatic dnmt3b expression in fed male being significantly higher. Gnrh3 and npvf expression in fasted male and IT expression in fed female were higher. Temperature experiments revealed that food intake increased at higher temperatures, and npvf and Orexin levels were elevated at 32°C and 18°C in males. However, no significant changes were observed in epigenetic-related genes in response to temperature variations. Conclusion: This study demonstrates how environmental elements like temperature and diet can have a substantial impact on guppy gene expression and phenotypic adaptation. The results of this study advance our knowledge of the molecular mechanisms underlying living things' flexibility and adaptation to environmental changes and may find use in aquaculture and conservation.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".