Additional file 1 of A mouse model of ATRX deficiency with cognitive deficits and autistic traits
Bibliographic record
Abstract
Additional file 1: Fig. S1. Validation of ATRX deletion in AtrxNEXCre female mice. A) ATRX immunofluorescence staining (red) of E13.5 control and AtrxNEXCre brain coronal cryosections. DAPI counterstaining is shown in blue. Scale bar, 570 μm. B) Magnified view of ATRX (top, red) and βIII-tubulin (bottom, green) staining in the developing cortical plate. Scale bar, 144 μm. C) ATRX (red) and DAPI (blue) staining in the cortex and hippocampus of AtrxNEXCre and control mice at P20. D) ATRX (red) and NeuN (green) staining in the cortex and hippocampus at P20. Cortex scale bar,100 μm and hippocampus scale bar, 400 μm. CA: cornu Ammonis, CP: cortical plate, Ctx: cortex, DG: dentate gyrus, GE: ganglionic eminence, LV: lateral ventricle, VZ: ventricular zone. eminence. Magnified images of cortical plate scale bar, 144 μm. VZ: ventricular zone, CP: cortical plate. Fig. S2. Spatial learning deficits in AtrxNexCre mice. A) Latency to find the platform during the training phase of the Morris water maze test shows that AtrxNEXCre male and female mice have impaired spatial learning (p<0.0001, F=3.630). B) AtrxNexCre mice spend less time swimming during the trials, reflecting their floating behaviour (p<0.0001, F=19.50) (Controlmale n=7, AtrxNEXCre male n=7, Controlfemale n=7, AtrxNEXCre female n=7). C) A 6-minute forced swim test revealed increased apathy in water for male mice (p=0.0292, F=2.028) (Controlmale n=15, AtrxNEXCre male n=12) and decreased apathy in female mice (p=0.0154, F=3.149, Controlfemale n=13, AtrxNEXCre female n=12). Sex discrimination index reveals sex differences in the forced swim test (p=0.0002, F=2.370). Error bars represent +/-SEM. Three-way ANOVA or Students t-test performed when appropriate (* p<0.05, ** p<0.001, ***p<0.0001). Fig. S3. Self injury and reduced olfaction in AtrxNEXCre mice. A) Example images of mice with evidence of self-injury caused by over-grooming. B) Male and female AtrxNEXCre mice spend less time sniffing the almond and banana odors during the olfaction test (p<0.0001, F=18.65; Controlmale n=17, AtrxNEXCre male n=14, Controlfemale n=13, AtrxNEXCre female n=13). C) Male and female mice of each genotype explored the three-chamber apparatus freely during the 10-minute habituation portion of the three-chamber assay (Controlmale: n=13, AtrxNEXCre male n=13, Controlfemale: n=13, AtrxNEXCre female n=11) (L= left side, C= center, R= right side). Error bars represent +/-SEM. Two-way Anova with Sidak post-hoc (*p<0.05, ** p<0.001, ***p<0.0001).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.009 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.002 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.843 | 0.118 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".