Video 1_Applications of synchrotron light in seed research: an array of x-ray and infrared imaging methodologies.mp4
Bibliographic record
Abstract
Synchrotron radiation (SR) provides a wide spectrum of bright light that can be tailored to test myriad research questions. SR provides avenues to illuminate structure and composition across scales, making it ideally suited to the study of plants and seeds. Here, we present an array of methodologies and the data outputs available at a light source facility. Datasets feature seed and grain from a range of crop species including Citrullus sp. (watermelon), Brassica sp. (canola), Pisum sativum (pea), and Triticum durum (wheat), to demonstrate the power of SR for advancing plant science. The application of SR micro-computed tomography (SR-µCT) imaging revealed internal seed microstructures and their three-dimensional morphologies in exquisite detail, without the need for destructive sectioning. Spectroscopy in the infrared spectrum probed sample biochemistry, detailing the spatial distribution of seed macronutrients such as lipid, protein and carbohydrate in the embryo, endosperm and seed coat. Methods using synchrotron X-rays, including X-ray absorption spectroscopy (XAS) and X-ray fluorescence (XRF) imaging revealed elemental distributions, to spatially map micronutrients in seed subcompartments and to determine their speciation. Synchrotron spectromicroscopy (SM) allowed chemical composition to be resolved at the nano-scale level. Diverse crop seed datasets showcase the range of structural and chemical insights provided by five beamlines at the Canadian Light Source, and the potential for synchrotron imaging for informing plant and agricultural research.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.074 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".