Additional file 1 of Metabolic switching, growth kinetics and cell yields in the scalable manufacture of stem cell-derived insulin-producing cells
Bibliographic record
Abstract
Additional file 1. Figure S1: Morphology, doubling times and representative flow cytometry plots during the first four stages of differentiation. (A) Representative morphology of the cell monolayer from S1D3 to S4D3, scale bar= 500 µm. (B) Quantification of stage-specific doubling time, where a negative value indicates a decline in the cell population (n= 13-15 biological replicates; box and whisker plots show individual points with median and interquartile range, one-way ANOVA with Tukey post-test). (C) Viability of undifferentiated ESCs (n= 22 independent replicates). (D) Representative flow cytometry plots of ES, S1D3 cells showing gating strategy using the appropriate isotype controls (blue). (E) Viability of lifted S4D3 cells (n= 22 independent replicates). (F) Representative flow cytometry plots of S4D3 and S4D4 cells showing gating strategy using the appropriate isotype controls (blue). (G) Aggregation efficiency of AggreWellTM vs. PBS-Mini. The lines indicate paired experiments using the same input S4D3 cells to seed both AggreWellTM and PBS-Mini (paired two-tailed t-test; n= 8 biological replicates note that one AggreWellTM point is repeated from Fig. 1K for comparison). (H) Morphology and diameter of S4D4 aggregates formed with PBS-Minis using the indicated seeding density, scale bar= 500 µm (n= 399-479 aggregates/group; plot shows individual aggregates with median and interquartile range). S1D3 = stage 1 day 3, S2D3 = stage 2 day 3, S3D2 = stage 3 day 2, S4D3 = stage 4 day 3, S4D4 = stage 4 day 4
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.017 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.004 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.003 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.800 | 0.197 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".