Additional file 1 of Metabolic switching, growth kinetics and cell yields in the scalable manufacture of stem cell-derived insulin-producing cells
Bibliographic record
Abstract
Additional file 1. Figure S1: Morphology, doubling times and representative flow cytometry plots during the first four stages of differentiation. (A) Representative morphology of the cell monolayer from S1D3 to S4D3, scale bar= 500 µm. (B) Quantification of stage-specific doubling time, where a negative value indicates a decline in the cell population (n= 13-15 biological replicates; box and whisker plots show individual points with median and interquartile range, one-way ANOVA with Tukey post-test). (C) Viability of undifferentiated ESCs (n= 22 independent replicates). (D) Representative flow cytometry plots of ES, S1D3 cells showing gating strategy using the appropriate isotype controls (blue). (E) Viability of lifted S4D3 cells (n= 22 independent replicates). (F) Representative flow cytometry plots of S4D3 and S4D4 cells showing gating strategy using the appropriate isotype controls (blue). (G) Aggregation efficiency of AggreWellTM vs. PBS-Mini. The lines indicate paired experiments using the same input S4D3 cells to seed both AggreWellTM and PBS-Mini (paired two-tailed t-test; n= 8 biological replicates note that one AggreWellTM point is repeated from Fig. 1K for comparison). (H) Morphology and diameter of S4D4 aggregates formed with PBS-Minis using the indicated seeding density, scale bar= 500 µm (n= 399-479 aggregates/group; plot shows individual aggregates with median and interquartile range). S1D3 = stage 1 day 3, S2D3 = stage 2 day 3, S3D2 = stage 3 day 2, S4D3 = stage 4 day 3, S4D4 = stage 4 day 4
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.926 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".