Development of a green fluorescent protein (GFP) expression vector for transformation of potato ring rot bacterium
Bibliographic record
Abstract
Potato bacterial ring rot caused by Clavibacter michiganensis subsp. sepedonicus (CMS) (formerly Corynebacterium sepedonicus), a gram-positive bacterium, is a big concern to seed and processing potato producers in Canada and worldwide. A plasmid, pHN216 (13.8 kbp), previously modified from two wild type plasmids isolated from C. michiganensis subsp. michiganensis (CMM) was used as a cloning vector. Two variants of jellyfish (Aquaria victoria ) green fluorescent protein (GFP) gene (gfp) were inserted in plasmid pHN216 in order to express GFP in CMS. In this study experiments were conducted to transform both CMS and CMM with the recombinant plasmid. Although CMS was the target of primary interest, transformation of CMM was also done. CMM is a pathogen of tomato and does not infect potato. In this study, two recombinant plasmids, pHNG and pHNCG were constructed. Escherichia coli, CMS and CMM cells were then transformed with these plasmids. A new method for the preparation of competent cells and for electroporation was developed in this study for enhancing the efficiency of transformation and all three bacterial species were successfully transformed with either pHNG or pHNCG. (Abstract shortened by UMI.).
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".