Functional validation of the genetic architecture of «Salmonella» persistence in 129S6 mice and the impact of Ses1 (Nramp1) on the transcriptome of «Salmonella» enteritidis during chronic carriage
Bibliographic record
Abstract
Salmonella Typhimurium and Salmonella Enteritidis cause a food-borne disease resulting in gastroenteritis. To study the persistence of Salmonella during the late phase of infection, a mouse model was developed using C57BL/6J mice that clear the bacteria completely from the spleen and lymph nodes within 42 days post-infection and 129S6 mice that become chronic carriers. Linkage analyses using a cross between C57BL/6J and 129S6 mice led to the mapping of ten quantitative trait loci, Ses1 to Ses10, affecting Salmonella persistence in mice. In the females, Ses3 showed significant effects on bacterial clearance and two significant interactions between Ses1.2 and Ses4 and between Ses1.2 and Ses5. To functionally validate in vivo the interaction between Ses1.2 and Ses4 or Ses5, we created double congenic mice. Bacterial counts in the spleen and the liver demonstrated that both the 129S6.B6-Ses1.2/Ses4 and the 129S6.B6-Ses1.2/Ses5 mice clear Salmonella Enteritidis more efficiently than 129S6 or single congenic mice at day 42 post-infection validating the interaction terms identified by statistical analyses. We also tested the candidacy of Hamp as the gene underlying Ses5 by quantitative complementation test in which we did not detect a significant interaction between Hamp and Ses5. In addition, the effect of Nramp1 on the Salmonella expression of virulence factors, notably mgtB and phoP was investigated ex vivo and in vivo. To validate our hypothesis in vivo, we present the creation of ÎmgtB and ÎphoPQ Salmonella Enteritidis mutants.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".