Gene activation potential of MsHsfA4, a heat shock transcription factor from Medicago sativa L.
Bibliographic record
Abstract
A class A4 heat shock transcription factor (Hsf) from 'Medicago sativa' L. was compared to a class A1 and a class A4 Hsf from ' Arabidopsis thaliana'. Transient expression assays with [beta]-Glucuronidase and Luciferase reporter genes were used in a replicated study comparing mesophyll protoplasts from five species, 'Nicotiana tobaccum, Arabidopsis, M. sativa, M. truncatula' and 'Lotus japonicus'. A significant Hsf protein by protoplast interaction (P=0.0162) was detected. The 'Arabidopsis' class A1 activated significantly (P<0.05) higher levels of reporter compared to the 'Arabidopsis' and ' Medicago' class A4 in 'Nicotiana' protoplasts. In ' Arabidopsis', and 'M. truncatula' and 'Lotus ' protoplasts it was the reverse; the A4 Hsf induced higher expression than the A1. The class A4 'Arabidopsis' and 'Medicago ' had comparable activities in 'M sativa, M. truncatula' and 'Lotus' protoplasts. This study revealed that model plant species have differential response which altered the relative activation of Hsf proteins in the heat shock response.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".