Spliced leader (SL) «trans»-splicing in the ascidian tunicate «Ciona intestinalis»: molecular characterization of the SL RNA
Bibliographic record
Abstract
I initially set out to identify the cap structure on the spliced leader RNA of Ciona intestinalis.During this investigation, I discovered a previously unobserved 53 nt transcript containing the spliced leader sequence.This transcript contained the usual metazoan spliced leader RNA cap, trimethylguanosine, while the canonical spliced leader RNA lacked this moiety, but likely contained a m 7 G cap.The cap structure of the trans-spliced troponin I mRNA matched that of the canonical spliced leader RNA, indicating that the canonical spliced leader RNA, not the 53 nt transcript, is the donor for troponin I. Further immunoprecipitation studies showed that both the canonical spliced leader RNA and the novel 53 nt transcript exist in association with Sm proteins.I cloned a genomic DNA segment containing four tandem repeats of the spliced leader RNA gene, and this was used as a probe in an in situ hybridization study that showed the vast majority of the spliced leader RNA genes reside on chromosome 8.Finally, I performed some preliminary work showing that outrons, the 5"segments of pre-mRNAs removed by trans-splicing, may exist in sufficient quantities as to be detected by PCR amplification.ABSTRAIT Au d"part, j'ai entrepris d"identifier la structure coiffe de l"ARN spliced leader de Ciona intestinalis.Durant cette recherche, j'ai dcouvert un nouveau transcript d"RN de 53 nt qui contient la squence spliced leader.Ce transcrit contient la coiffe usuele des ARNs spliced leader des mtazoaires, le trimethylguanosine, tandis que l"ARN spliced leader canonique ne possde pas cette modification, mais contient, fort probablement, une coiffe m 7 G. La structure de la coiffe de l"ARNm de la troponine I trans-piss correspond celle de l"ARN spliced leader canonique, indiquant que l"ARN spliced leader est le donneur pour la troponine I, et non l"ARN de 53 nt.Des tudes d'immunoprcipitation supplmentaires ont montr que l"ARN spliced leader canonique et le nouvel ARN de 53 nt existent en association avec des protines Sm.J'ai clon une sequence d"ADN gnomique contenant quatre repetitions en tandem du gne l"ARN spliced leader.Ce clone a t utilis comme sonde lors d"une experience d"hybridation in situ qui a montr que la grande majorit des gnes l"ARN spliced leader rside sur le chromosome 8.Finalement, j'ai effectu une tude prliminaire montrant que les outrons, les segments 5' des ARNs pr-messagers enlevs par le trans-pissage, existent en quantit suffisante et peuvent tre dtect par l'amplification PCR.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".