The architecture of the ERI complex involved in initiating endogenous RNA interference in «C.elegans»
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
RNA interference (RNAi) is a gene silencing mechanism occurring in a wide variety of organisms.Different RNAi pathways exist in C. elegans for the regulation of various endogenous processes.The ERI-mediated RNAi pathway involves the ERI proteins and C. elegans Dicer, DCR-1, suggested to be involved in initiating endogenous RNAi.In this study, it was observed that many of the ERI proteins co-fractionate with DCR-1 in a complex of ~885 kDa in size.It was shown that the RdRP RRF-3 and the Tudor domain protein ERI-5 require each other in order to associate with DCR-1, but independently of ERI proteins ERI-1 and ERI-3.Furthermore, it was also observed that the helicase DRH-3 requires RRF-3 and ERI-5 in order to associate to DCR-1.The data suggested that at least two distinct binding sites for ERI proteins are found on DCR-1.Preliminary GST pulldown data confirmed distinct binding regions on DCR-1 for ERI-5 and ERI-3. III RsumL"ARN interference (RNAi) est un mcanisme de "silencing" de l"expression gnique se produisant chez de nombreux organismes.Chez C. elegans, il existe diffrentes voies RNAi rgulant de nombreuses fonctions biologiques.La voie dpendant d"ERI implique les protines de la famille ERI et DCR-1, l"orthologue de Dicer chez C. elegans, qui pourraient tre engages dans l"initiation du processus RNAi endogne.Durant mon Master, j"ai pu observer que beaucoup de protines ERI co-fractionnent avec DCR-1 au sein de complexes de 885kDa.La protine RdRP RRF-3 et ERI-5, une protine possdant deux domaines Tudor, sont interdpendantes pour l"association avec DCR-1 mais de manire indpendante d"ERI-1 et ERI-3.En outre, j"ai pu galement dmontrer que l"hlicase DRH-3 requiert RRF-3 et ERI-5 pour se fixer DCR-1.L"ensemble de ces donnes suggre qu"il existe au moins deux sites de fixation aux protines ERI sur la protine DCR-1.Enfin, mes rsultats prliminaires de GST-pulldown semblent confirmer la fixation d"ERI-3 et ERI-5 au niveau de rgions distinctes de DCR-1.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.003 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it