The mirB ferrisiderophore transporter of Aspergillus fumigatus: Structural requirements for activity
Bibliographic record
Abstract
Siderophores (iron chelators) have been identified as virulence factors in the opportunistic fungal pathogen, Aspergillus fumigatus (Afu). The 14-pass transmembrane protein MirB is postulated to function as a siderophore-transporter, responsible for uptake of the siderophore TAFC. The aim of my research was to identify amino acids in the extracellular loops of Afu MirB that are crucial for uptake of Fe-TAFC. Site-directed mutagenesis was used to create MirB mutants and expression of the WT and mutant proteins in Saccharomyces cerevisiae strain PHY14 was confirmed by western blotting. TAFC transport assays using 55Fe-labelled TAFC and growth assays with Fe-TAFC as the sole iron source identified alanine 125, tyrosine 577, loop 3 and the second half of loop 7 as crucial for function, since their substitution or deletion abrogated uptake completely. This study has revealed critical residues important for TAFC uptake which could represent potential targets for the development of novel antifungals.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".