The role of transcription and splicing on histone H3 lysine 4 trimethylation dynamics
Bibliographic record
Abstract
Histone H3 lysine 4 trimethylation (H3K4me3) is a histone post-translational modification that is normally located near the transcription start site and first 5’ splice site of a gene. Enzymes are involved in regulating the addition and removal of H3K4me3 along the length of the gene body. However, little is understood about these changes during transcriptional induction. Additionally, the effects of splicing inhibition have not been examined in an endogenous system. We hypothesized that transcription and pre-mRNA splicing at the 5’ coding region plays a role in H3K4me3 dynamics. Chromatin immunoprecipitation assays ± splicing (isoginkgetin) or transcription (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) inhibitors were done to determine the H3K4me3 levels along the immediate early gene Fos. The gene was stimulated with epidermal growth factor, and monitored at various times. H3K4me3 levels increased and decreased along the induced Fos gene. Inhibition of transcription or splicing prevented these changes in H3K4me3 levels during transcription, suggesting that splicing may influence H3K4me3 levels indirectly. The significance of these finding is that it provides a better understanding of how H3K4me3 is regulated during transcription and splicing of a gene.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".