Social isolation induced behavioural and microstructural differences in juvenile mice
Bibliographic record
Abstract
Myelin is a lipid-rich molecular bilayer that supports neuron function, stabilizes axons, helps synchronize neurotransmission, and remains plastic throughout life.The relationship between experience and adaptive myelination is best studied using animal models.Social isolation (SI) is a deprivation paradigm that elicits hypomyelination in the prefrontal cortex (PFC) and behaviour deficits including impaired sociability and increased anxiogenic behaviour.Implications of SI during the critical period of PFC development in mice was investigated with the goal of reproducing results of previous studies, exploring sex differences in vulnerability, and determining the ability of quantitative magnetic resonance imaging (MRI) to detect small differences in myelin in vivo.60 (28 males) C57BL/6J mice were weaned at postnatal day 21, and housed in SI or as social controls for two weeks.Behaviour tests were conducted for sociability and anxiogenic behaviour, and in 48 mice (24 males) MRI scans were performed in vivo on a small bore 7 tesla scanner.Magnetization transfer ratio (MTR) and MT saturation (MTsat) maps were calculated using three FLASH images at 200µm isotropic resolution to estimate brain myelin content.MTweighted images were used to detect local brain volume differences between groups using deformation based morphometry (DBM) analysis.SI of male mice led to increased anxiogenic behaviour (p=0.02)compared to social controls.There was no difference in male or female sociability, or in female anxiogenic behaviour.DBM analysis did not reveal local anatomical differences between groups.The lack of variation in MTsat suggests there was little to no SI-induced hypomyelination in the PFC after two weeks of SI.MTR was increased in isolated males compared to controls in the limbic stress différences anatomiques locales entre les groupes.L'absence de différence entre les groupes dans MTsat suggère qu'il y a peu ou pas d'hypo-myélinisation induite par le SI.La MTR a augmenté chez les mâles isolés par rapport aux contrôles dans le circuit de stress limbique (hippocampe et amygdale).Il n'y avait pas de relation significative entre le comportement et les résultats de la MTR.Le SI après le sevrage produit une cascade d'événements qui conduisent à une altération de la plasticité synaptique et de la densité dendritique dans les zones limbiques.Cela explique probablement l'augmentation observée du comportement anxiogène des mâles.Les souris femelles sont plus résistantes à le SI, mais les données préliminaires pour une période SI de 4 semaines suggèrent que cela peut être une relation dose-dépendante.Des études antérieures montrent qu'une période plus longue de SI conduit à un déficit en myéline et à un phénotype comportemental plus prononcés.Une période prolongée de SI est nécessaire pour évaluer la sensibilité du MRI à l'hypo-myélinisation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".