Thermotolerance in «dipsosaurus dorsalis»: insights into protein thermostability at the primary sequence level
Bibliographic record
Abstract
Protein stability in solution is often a limiting factor in certain biochemical and structural biology studies. Proteins from the thermotolerant vertebrate Dipsosaurus dorsalis, the desert iguana, have been studied as a possible means to provide stable homologues of mammalian proteins of interest, notably the first nucleotide-binding domain (NBD1) of the cystic fibrosis transmembrane conductance regulator (CFTR). Cystic fibrosis (CF) is an often fatal human disease, which is caused by the malfunction of CFTR. The vast majority of CF patients have a common mutation: a deleted phenylalanine residue (F508) located in the NBD1 domain. The genes coding for the desert iguana NBD1 (iNBD1) and the C-terminal domain of poly-A binding protein (iPABC) were both cloned by reverse transcription polymerase chain reaction (RT-PCR) of RNA purified from heart tissue. The human and iguana PABC amino acid sequence was found to be identical. Comparison of the iguana NBD1 amino acid sequence to human showed that four amino acid substitutions known to solubilize human NBD1 were naturally occurring in the desert iguana. These residues are localized on a solvent-exposed face of the domain. Sequence analysis of published genomes showed that these solubilizing residues are not unique to the desert iguana. The iNBD1 protein was purified as a GST fusion and an HSQC spectrum was recorded. This analysis offers preliminary data to drive and direct future structural biology studies using thermostable proteins from D. dorsalis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".