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Record W6999223465

Characterization of a carbon source-dependent, phytohormone-responsive, free methionine-(R)-sulfoxide reductase from Komagataeibacter hansenii

2021· dissertation· en· W6999223465 on OpenAlexaff

Bibliographic record

Venuee-scholar@UOIT (University of Ontario Institute of Technology) · 2021
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMicrobial metabolism and enzyme function
Canadian institutionsOntario Tech University
Fundersnot available
KeywordsReactive oxygen speciesMethionine sulfoxide reductaseBacterial celluloseOxidative stressMutantExtracellularSugarBacteriaBiosynthesis
DOInot available

Abstract

fetched live from OpenAlex

Komagataeibacter hansenii ATCC 23769 is a gram negative, non-pathogenic, acetic acid bacterium, best known for its ability to produce bacterial cellulose (BC). BC, an extracellular polysaccharide, is an important component of biofilms as it promotes anchoring to surfaces for nutrient acquisition and protection from environmental stress. BC has been long studied however, there is a lack of research pertaining to the regulation of BC production. In this thesis, the functional role of a putative free methionine sulfoxide reductase (MsrC) was characterized using a knockout mutant. The growth of the mutant was greatly affected by the presence of ethylene, abscisic acid and indole-3-acetic acid while oxidative stress increased BC production. Fructose-grown cultures produced more reactive oxygen species than in glucose. Oxidative stress upregulated genes involved with survival, growth, cellulose production, and stress defense. Therefore, MsrC is both phytohormone and oxidative stress responsive, coordinating cellular functions such as growth and BC production.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Research integrity
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.330
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.187
Teacher spread0.181 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2021
Admission routes1
Has abstractyes

Explore more

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