Polydopamine nanoparticles in the treatment of autosomal recessive spastic ataxia of Charlevoix-Saguenay
Bibliographic record
Abstract
The purpose of this work was to evaluate the therapeutic effect of antioxidant polydopamine nanoparticles (PDNPs) for the treatment of autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disorder attributed to a mutation of the gene responsible for the production of sacsin, a protein involved in mitochondrial network organization. The first part of the thesis was focused on the fabrication of PDNPs and their characterization in terms of morphology, hydrodynamic diameter, z-potential, and antioxidant activity. We tested PDNPs on different in vitro models like SH-SY5Y, hCMEC/D3, primary human astrocytes, and primary human fibroblasts, evaluating their effects on cell viability and internalization. We also assessed the ability of the nanoparticles to stimulate neurite outgrowth and neuronal differentiation. The ability of PDNPs to counteract reactive oxygen species (ROS) production upon pro-oxidative stimuli in different in vitro models was assessed through flow cytometry. Finally, PDNPs ability to prevent ROS induced damage upon mitochondria was evaluated in neuronal-like cells derived from differentiated SH-SY5Y, primary human fibroblasts from a healthy donor, and primary human fibroblasts from a patient affected by ARSACS.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".