Investigating the genetic basis of germline quiescence in «C. elegans» dauers
Bibliographic record
Abstract
When faced with unsatisfactory environmental conditions, young C. elegans larvae can enter a diapause-like stage called dauer. The decision to form dauer is controlled by three parallel signalling pathways (TGF-β, cGMP and insulin/IGF-like signalling) that converge on DAF-12, a nuclear hormone receptor that prompts either dauer formation or reproductive growth depending on the environmental cues presented to C. elegans during its development. DAF-12 is related to the Vitamin D, Liver-X, and Androstane receptors, and similar to these human NHRs, responds to cholesterol-derived hormones. Ligand-bound DAF-12 has transcriptional activity directing reproductive development, while unliganded DAF-12 forms a dauer-specifying complex that regulates the transcription of genes required for dauer development with its interacting protein DIN-1S. I report here that din-1S is required in the germ cells upstream of insulin-like signalling (IlS) and in parallel to LKB1/par-4 signalling to establish dauer germline quiescence. I also observed that din-1S is important for post-dauer reproductive fitness when IlS is impaired. Moreover, I demonstrate that din-1S is necessary for long dauer life span in response to reduced insulin signalling, either downstream of, or in parallel to, AMPK/aak-2. Finally, I show that din-1S acts upstream of IlS pathway components to restrict somatic gonad precursor cell proliferation during dauer development, and uncover a conditional role for LKB1/par-4 signalling in this process.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".