Functional and structural characterization of cytoplasmic poly(A) binding protein
Bibliographic record
Abstract
Cytoplasmic poly(A) binding protein (PABP) binds the 3' poly(A) tail of mRNA and plays an important role in translation of genetic information.Here, I review our knowledge of PABP with a focus on the C-terminal MLLE domain, and present the results of functional and structural studies of PABP.I elucidated the interactions between PABP and its binding partner Paip2.These interactions could regulate PABP and potentially affect PABP dissociation from mRNA.I found a link between PABP and formation of P-bodies, which suggests that PABP is important in mRNA decay and remodeling of mRNA-protein complexes.I also identified a new PAM2 motif in RNArelated LARP4.Structural characterization revealed a conserved pattern of residues in the PAM2 motif and enabled us to look for additional PAM2-containing proteins.Finally, I report a study of PABP isoform specific functions.The study utilized new genome editing technology and RNA-seq analysis to detect transcriptome changes that correlate with PABP isoform usage.In all, the thesis presents multidisciplinary studies of PABP function and structure.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".