Tension at the leading edge: differential expression of the cell adhesion molecule Echinoid controls epithelial morphogenesis in «Drosophila»
Bibliographic record
Abstract
Epithelial morphogenesis requires cell shape changes coordinated by local modulations of the actin cytoskeleton. We identify a role for Echinoid (Ed), a homophilic binding cell adhesion molecule, in the generation of contractile actomyosin cables that drive epithelial morphogenesis in both the Drosophila ovarian follicular epithelium and embryo. Specifically, we demonstrate that such contractile structures form at the interface of cells expressing and lacking Ed. Analysis of such interfaces generated by ed mutant follicle clones indicates that the juxtaposition of wild type and ed mutant cells results in the asymmetric localization of Ed in the wild type cell, which is sufficient to trigger the formation of an actomyosin cable at the clone interface. In wild type ovaries and embryos, specific cell types lack Ed, thus creating endogenous interfaces between cells with and without Ed; these interfaces display the same contractile cable as Ed interfaces created by ed mutant clones. In the ovary, this boundary lies between the two cell types of the dorsal appendage primordia. In the embryo, the absence of Ed from the amnioserosa during dorsal closure generates an Ed expression interface with the lateral epidermis, which coincides with the well-characterized actomyosin cable present in the epidermal cells at this interface. In both cases, elimination of Ed leads to the loss of the actomyosin cable causing subsequent defects in morphogenesis. Furthermore, we found that the asymmetric distribution of Ed in cells abutting Ed non-expressing cells is essential to polarize the actin regulators Diaphanous, Enabled and RhoGEF2 and downregulate the polarity protein Bazooka at the face of the cell where the actomyosin cable assembles. We conclude that the asymmetric distribution of Ed polarizes the actin cytoskeleton thus triggering the local and coordinated assembly of actomyosin cables at differential Ed interfaces. Disruption of the asymmetric distribution of Ed by e
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".