The in vitro culture and regeneration of elite Canadian barley genotypes
Bibliographic record
Abstract
In order to use tissue culture to improve elite Canadian genotypes of barley ('Hordeum vulgare' L.), current tissue culture protocols require revision. Three studies were conducted to evaluate the effect of genotype, the composition of the initiation media, effect of explant preparation, the composition of the regeneration media and the location in which the donor plants were grown on the success of barley embryo culture. "AC Bacon", "AC Metcalfe" and "Oxbow" were the elite Canadian cultivars evaluated in the field and growth cabinet studies, and the cultivar "Golden Promise" was also included as it is regarded as the model cultivar in barley tissue culture systems. The greenhouse study (Section 4.0) evaluated the same cultivars except "AC Metcalfe" was replaced by "AC Rosser". Immature zygotic embryos were used as the explant tissue for all studies. Explant preparation was evaluated by comparing intact immature embryos to microsections of immature embryos as the initial explant. Immature embryos collected from donor plants grown in the greenhouse, field plot or growth cabinet were compared as explants. Callus initiation media and regeneration media were all modified types of Murashige and Skoog (MS) media (Murashige and Skoog, 1962). (Abstract shortened by UMI.)
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".