Expansion of the CD8 memory T cells : implications for the self-renewal gene Hoxb4
Bibliographic record
Abstract
CD8 memory T (Tm) cells provide life long protection against recurrent infections and are maintained through self-renewal mechanisms.Hematopoietic stem cells (HSC) also possess the capacity to slowly self-renew assuring their long-term maintenance.Both cell types use the bone marrow as their preferred proliferation niche.CD8 Tm and HSC partially share a transcriptional profile including some genes known to control self-renewal.Hox genes are expressed in HSCs, among them Hoxb4 is a potent enhancer of HSC expansion in vitro and in vivo.Based on the similarities between HSC and Tm cells, we hypothesize that genes involved in HSC self-renewal, like Hoxb4, will promote CD8 Tm cell expansion.To test this hypothesis, we have determined the effect of Hoxb4 overexpression in T cells from young and old transgenic mice, and on the engraftment and maintenance of CD8 Tm cells after transplantation into immunocompetent or immunodeficient mice.At the steady-state, the composition and phenotype of the naïve and memory T cells were not affected by Hoxb4 overexpression.To study CD8 Tm cell maintenance, the Hoxb4 and the OT-1 transgenic mice were crossed; the latter express a T cell receptor that recognizes the ovalbumin (OVA) peptide, presented by major histocompatibility complex class I. Using this model, we generated in vitro large numbers of monoclonal and OVA specific CD8 Tm cells for adoptive transfer experiments.The number and the phenotype of Tm cells generated in culture were not affected by Hoxb4 overexpression.These cells were transplanted into wild type mice to evaluate their engraftment and long-term maintenance.After transplantation in either immunocompetent or immunodeficient mice, Hoxb4 overexpressing Tm cell engraftment or maintenance was not enhanced when compare to wild-type Tm cells.These results demonstrate that Hoxb4 does not promote CD8 Tm cell self-renewal.Further studies will try to identify the factors controlling CD8 Tm cell self-renewal.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".