RNA binding protein SRRM 2/3/4 is required for e2f1b splicing in Drosophila
Bibliographic record
Abstract
E2f1 is a transcription factor necessary for the regulation of cell cycle related checkpoints and activities.Drosophila melanogaster is known to have two activator e2f1 isoforms, produced by alternative splicing: e2f1a and e2f1b.E2f1b contains a 16aa microexon (termed 3b) in the MB domain that is spliced via unknown alternative splicing mechanisms.In this thesis, I report my studies on neural RNA binding protein SRRM 2/3/4 as a regulator and promoter of e2f1b specific splicing.Studies were carried out in larval tissues using molecular methods such as qPCR, immunofluorescence, in situ hybridization, RNA interference, and confocal microscopy to better understand the scope of SRRM 2/3/4's regulatory influence on alternative splicing of e2f1.My data suggests that SRRM 2/3/4, although not majorly involved in the salivary gland as opposed to neural tissues, promotes inclusion of micro exon 3b in the salivary gland.Additionally, srrm 2/3/4 RNAi salivary glands are marked by disrupted cell cycles in srrm 2/3/4 RNAi salivary glands, diminished PCNA-GFP activity, which recapitulates many aspects of the e2f1b-deficient salivary glands.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".