Structural analyses of the putative mannosylphosphate transferase, Ktr6p from «Saccharomyces cerevisiae»
Bibliographic record
Abstract
The post-translational addition of sugar moieties onto proteins plays an important role in protein folding, secretion, signaling, and is even thought to partake in cell-cell adhesion.In eukaryotes, the biosynthesis of N-glycans begins with the transfer of Glc 3 Man 9 GlcNAc 2 to the amide group of an asparagine situated in the tripeptide Asn-X-Thr(Ser), of a protein.Subsequent removal of three glucoses and one mannose occurs in the Endoplasmic Reticulum.In yeast, those proteins that require further maturation, e.g.those destined to enter the secretory pathway, are sent to the cis-Golgi.The core, Man 8 GlcNAc 2 , is then decorated with additional mannoses and mannose-1-phosphates.The enzyme responsible for the transfer of mannose-1-phosphate in Saccharomyces cerevisiae is Ktr6p.It is a member of the Kre Two Related family of mannosyltransferases: Kre2p.Ktr6p shares 38% sequence identity with Kre2p, suggesting a similar three-dimensional structure.However, greater than 50% of the residues in the Ktr6p putative active site differ from Kre2p, signifying that Ktr6p may have a modified mannosyltransferase function.For these aforementioned reasons, crystallographic studies on this putative mannosylphosphate transferase are pursued.Here, we present the methods employed to obtain the final Ktr6p crystal structure that has been solved, via molecular replacement, to a resolution of 3.11 .A detailed discussion analyzing the results obtained ensues.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".