Searching for HES6 dimerization partners
Bibliographic record
Abstract
HES6 is a novel member of the basic helix-loop-helix (bHLH) family of transcription factors that is involved in the activation of the myoblast differentiation program. Studies demonstrated that HES6 can regulate transcription from gene promoters containing an N box (CACNAG), a DNA motif located in the regulatory regions of many muscle-specific genes involved in myoblast differentiation. This suggested that a HES6 heterodimer could bind to the N box. The purpose of this study was to identify putative HES6 dimerization partners that would allow it's binding to the N box and then repress transcription. A genome-wide screen isolated several clones. Potential positive clones, the heat shock proteins HSP169 and HSP70 were identified. The functional significance of this interaction remains to be determined. A candidate gene approach allowed detection of an interaction in vivo between HES6 and a novel hairy-related bHLH protein, CHF2. This interaction was detected by co-immunoprecipitation using the first specific anti-HES6 antibody synthesized. Electrophoretic mobility shift assays revealed that the heterodimer was unable to bind to any of the N-boxes present in the 5'-flanking region of CHF2, which was confirmed by assessing the specificity of the controls used in the experiments. It was suggested that HES6 sequesters CHF2 by the formation of an inactive heterodimer. Although a band shift was observed when incubating each of the Nboxes in the presence of total cell extract from differentiated C2C12 cells, it was not confirmed whether HES6 was one of the proteins binding to the oligonucleotides. This work has identified a novel interaction between HES6 and CHF2 and our data suggests that this interaction lead to formation of inactive heterodimers.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.005 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".