Block copolymer micelles and vesicles : loading, release and cellular internalization with applications to drug delivery
Bibliographic record
Abstract
This thesis describes the preparation, physico-chemical characterization and pharmacological studies of block copolymer micelles and vesicles for drug delivery applications. The incorporation and partition coefficients of model hydrophobic compounds (benzo[a]pyrene and Cell Tracker-DiI) are investigated in polycaprolactone-block-poly(ethylene oxide) copolymer micelles (PCL-b-PEO). The release of the probes is also studied from PCL-b-PEO micelles under perfect sink conditions. To investigate the usefulness of PCL-b-PEO micelles as a delivery vehicle for hydrophobic drugs, 17beta-estradiol (E2) is incorporated and released from the micelles. The biological activity of E2 is retained after micelle preparation and delivery to C57BL female mice. To investigate the cellular internalization of block copolymer micelles, gold labeled poly (4-vinylpyridine)- block-poly(ethylene oxide) micelles are internalized into A549 lung cells and HEK 293 kidney cells. The gold labeled P4VP-b-PEO micelles are visualized by transmission electron microscopy inside of endosomes and lysozomes of these cells. The incorporation and release of a hydrophilic drug, doxorubicin hydrochloride (DXR), into polystyrene-block-poly(acrylic acid) vesicles are investigated. An active pH loading method is used to incorporate DXR into the aqueous center of the vesicle. A plasticizer, dioxane is used to control the loading and release of DXR by tuning the permeability of the vesicle walls.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".