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Record W7054677728

Antimicrobial activity and mechanism of allyl isothiocyanate action against bacteria

2016· dissertation· en· W7054677728 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueMspace (University of Manitoba) · 2016
Typedissertation
Languageen
FieldEngineering
TopicMagneto-Optical Properties and Applications
Canadian institutionsUniversity of WinnipegUniversity of Manitoba
Fundersnot available
KeywordsSinigrinMyrosinaseAllyl isothiocyanateGlucosinolateAntimicrobialBacteriaIsothiocyanate
DOInot available

Abstract

fetched live from OpenAlex

Work was undertaken to examine the antibacterial mechanism of allyl isothiocyanate (AITC), as well as explore the hydrolysis of sinigrin by bacteria and sinigrin metabolism in Escherichia coli O157:H7. Glucosinolates are found in cruciferous plants and react with the plant enzyme myrosinase to form antimicrobial compounds called isothiocyanates (ITCs). The mechanism of bactericidal action of ITCs is not well understood. Inhibition experiments against 12 bacteria including foodborne pathogens and starter cultures indicated that AITC exhibited a minimum inhibitory concentration (MIC) profile most similar to antibiotics ciprofloxacin and polymyxin B. Similarities in mechanisms of action between groups were identified. The sinigrin degradation capacity of a range of bacteria was examined. The effect of glucose in combination with other sugars on the sinigrin degradation capacity of E. coli O157:H7 was assessed. No statistically significant differences in sinigrin utilisation were observed. Results indicated that the metabolism of sinigrin was simultaneous with glucose consumption.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.161
Threshold uncertainty score0.995

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.183
Teacher spread0.173 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it