Biofilms of Listeria monocytogenes produced at 12ºC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
Bibliographic record
Abstract
The biofilm-forming ability of 21 Listeria monocytogenes isolates, previously pulsotyped and corresponding to 16 strains, from different origins was evaluated using the Calgary Biofilm Device R , at 37 C. Biofilms of 4 selected strains were also produced either on pure cultures or on co-cultures with Pseudomonas aeruginosa (PAO1), at 12 C and at 37 C. For these biofilms, the minimum biofilm eradication concentrations (MBECs) of 4 commercial dairy sanitizers (1 alkyl amine acetate based-T99, 2 chlorine based-T66 and DD, and 1 phosphoric acid based-BP) were determined.Listeria monocytogenes biofilms grown, either at 37 C or 12 C, were able to achieve similar cell densities by using different incubation periods (24 h and 7 d, respectively).In co-culture biofilms, P. aeruginosa was the dominant species, either at 37 C or at 12 C, representing 99% of a total biofilm population of 6 to 7 log CFU/peg.Co-culture biofilms were generally less susceptible than L. monocytogenes pure cultures.More interestingly, the biofilms produced at 12 C were usually less susceptible to the sanitizers than when produced at 37 C. Single or co-culture biofilms of L. monocytogenes and PAO1, particularly produced at 12 C, retrieved MBEC values for agents T99 and BP that were, at times, above the maximum in-use recommended concentrations for these agents.The results presented here reinforce the importance of the temperature used for biofilm formation, when susceptibility to sanitizers is being assessed.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.006 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".