The Characterization of Baxb, a Splice Variant of the Pro-Apoptotic Bax and Protein
Bibliographic record
Abstract
Baxa is a pro-apoptotic member of the Bcl-2 protein family that regulates a key point in the control of apoptosis. The bax RNA undergoes a complex pattern of RNA splicing, with eight splice isoforms known to date. The next most abundant isoform to Baxa is Baxp, which has a unique carboxyl-terminal sequence and consequently lacks the transmembrane domain of Baxa. This study characterized Baxp as part of a larger project aimed at deducing the role of cellular localization in Bax protein function. A transient transfection assay was designed to determine the cell death activity of a protein in adherent cells. Baxp induced cell death to a greater extent than Baxa when transiently expressed in NIH 3T3 cells. The levels of Baxp expression were always low, regardless of the cell type or transfection method used. Additionally, Baxp adopted a conformation in which amino acids 13 to 19 are accessible to the monoclonal antibody 6A7. In Baxa this epitope is only exposed in the membrane-bound activated conformation that is associated with accessibility of the BH3 domain of Baxa that mediates protein-protein interactions among Bcl-2 family members. Although the mechanism remains elusive, Baxp was identified as a potent inducer of cell death.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".