A forward genetic screen for Caenorhabditis elegans pharyngeal gland cell defects and characterization of mutagenized strains with a homozygous viable gland cell under migration
Bibliographic record
Abstract
Caenorhabditis elegans is a powerful genetic tool to study development. The pharynx is a neuromuscular organ of the upper digestive tract used to study how cells regulate their shape and movements during development. In the embryo, a receptor tyrosine kinase-like receptor (ROR), cam-1, is necessary for a single dorsal gland cell to migrate through the pharynx. A mutagenesis screen to isolate factors that may work with cam-1 examined 4,986 haploid genomes and isolated 60 strains with gland cell defects. Snip-SNP mapping placed one strain on the first chromosome, seven strains on the fourth, and one strain on the X chromosome. Complementation crosses and whole genome sequencing identified the LG IV mutations as cwn-2, a Wnt ligand, and ham-1, a storkhead box factor. The mutations on LG I and X require further testing to confirm the causative lesion. ham-1 RNAi in the cam-1 null background suggests that ham-1 and cam-1 function in different genetic pathways.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".