The Impact of Selected Gene Mutations to the Activation and DNA Binding Activity of the Transcription Factor VraR
Bibliographic record
Abstract
VraSR two-component system in S. aureus senses bacterial cell wall damage caused by antibiotics (vancomycin or -lactams) and regulates a set of genes. We have undertaken the study of the role of certain amino acids in the function of VraR, which undergo mutation in clinical S. aureus resistant strains and analyzed their effects on the VraSR signal transduction mechanism. In particular, we have looked at the role of Met-13 in VraR phosphorylation induced activation. M13A failure to be activated by phosphorylation resulted in a weaker binding to DNA. Moreover, we investigated VraR E59D, S164P, A113V, and S164A binding activity to the PvraSR by DNase-I footprinting. Finally, in vivo studies on the effects of substitutions of the above residues on the mRNA level of a number of VraSR regulon genes (fmtA, pbp2, and sgtb) provide insights as to how substitutions at certain positions may be beneficial to the S. aureus resistance to antibiotics.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".