PCR-RFLP PARA LA DETECCIÓN Y CARACTERIZACIÓN DE CIRCOVIRUS PORCINO TIPO 2 (PCV2) EN GRANJAS VENEZOLANAS
Bibliographic record
Abstract
El circovirus porcino tipo 2 (PCV2: Por sus siglas \nen inglés) se ha reconocido como el agente causal d\nel\nsíndrome multisistémico de emaciación postdestete (\nPMWS, por sus siglas en inglés; Postweaning\nmultisystemic wasting syndrome). El PMWS fue primer\no descrito en Canadá y reportado como enfermedad\nemergente en granjas de cerdos de todo el mundo. En \nVenezuela esta enfermedad fue diagnosticada en\npiaras de la región central del país en el año 2003\n. Con la finalidad de determinar la presencia del P\nCV2\nen nuestro país, 18 muestras de ganglio inguinal su\nperficial de cerdos con síntomas compatibles con\nPMWS provenientes de los estados Aragua, Lara y Yar\nacuy, fueron analizadas por una reacción en cadena\nde la polimerasa y análisis de polimorfismo del fra\ngmentos de restricción (PCR - RFLP: por sus siglas \nen\ninglés) específica para el marco de lectura abierto\n dos (ORF 2: por sus siglas en inglés) de PCV2. Tod\nas\nlas muestras de tejidos provenientes de cerdos con \nsíntomas de PMWS (18/18) resultaron positivas a\nPCV2 por PCR y la identidad genética de estos virus\n fueron determinada por secuenciación de los\namplicones. Usando una RFLP específica para el ORF2\n del PCV2, se encontró que en las granjas\nanalizadas circulan los subgenotipos 2A y 2D. Este \nestudio constituye el primer reporte de detección y\ngenotipaje de PCV2 en Venezuela utilizando un ensayo\n de PCR - RFLP.br\nPalabras claves del Autor: Circovirus tipo 2, Genotipo, Venezu\nela, RFLP.brbr\n\nABSTRACTbr\nPorcine circovirus type 2 (PCV2) has been recognize\nd as the causative agent of post-weaning multisyste\nmicwasting syndrome (PMWS). PMWS was first described i\nn Canada and reported as an emerging disease in\npig farms worldwide. In Venezuela the disease was d\niagnosed in herds of the midwest in 2003. In order \nto determine the presence of PCV2 in our country, 18 s\namples of superficial inguinal lymph of pigs with\nsymptoms consistent with PMWS from Aragua, Lara and\n Yaracuy states, were analyzed by Polymerase\nChain Reaction and Restriction Fragment Length Poly\nmorphism (PCR - RFLP) specific for Open Reading\nFrame 2 (ORF 2) of PCV2. All samples of tissues fro\nm pigs with PMWS symptoms (18/18) were positive for\nPCV2 by PCR. Using a RFLP analysis specific for ORF\n2 PCV2, the sub-genotypes 2A and 2D were found\ncirculating in the sampled farms. This study is the\n first report of detection and genotyping of PCV2 i\nn\nVenezuela using a PCR - RFLP assay.br\nKey words: Type 2 circovirus, Genotype, Venezuela, \nRFLPbrbr
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".