Regulation of the peripheral myelin protein-22 gene
Bibliographic record
Abstract
0-612-98341-2 L'auteur a accord une licence non exclusive permettant la Bibliothque et Archives Canada de reproduire, publier, archiver, sauvegarder, conserver, transmettre au public par tlcommunication ou par l'Internet, prter, distribuer et vendre des thses partout dans le monde, des fins commerciales ou autres, sur support microforme, papier, lectronique et/ou autres formats.L'auteur conserve la proprit du droit d'auteur et des droits moraux qui protge cette thse.Ni la thse ni des extraits substantiels de celle-ci ne doivent tre imprims ou autrement reproduits sans son autorisation.Conformment la loi canadienne sur la protection de la vie prive, quelques formulaires secondaires ont t enlevs de cette thse.Bien que ces formulaires aient inclus dans la pagination, il n'y aura aucun contenu manquant.to everyone suffering from a myeUn disorder 1-14 Objectives and rationale Chapter 2: General Materials and Methods 2-1 8 kb promoter construct 2-2 Cloning mou se Pmp22 gene 2-3 21 kb promoter construct 2-4 Recombination on BAC 100 kb gene construct 2-5 Targeting Pmp22/LacZ to hprt locus 2-6 Transgenesis Pronuclear injection, ES ceU recombination 2-7 Genotyping oftransgenic animals 2-8 Chloramphenicol Acyl transferase (CAT) Assay 2-9 j3-Galactosidase Assay 2-10 j3-Galactosidase Histochemistry 2-11 Sciatic nerve crush in jury 2-12 Southern Blot analysis 2-13 RT-PCR -14 Sequence comparisonsChapter 3: The expression of -8.5 kb promoter construct 3-1 Production and Characterization oftransgenic mice4 3-2 -8.5 kb of 5'flanking Pmp22 promoter targets reporter expression preferentially to peripheral nerve and Schwann ceUs.3-3 Expression of the -8.5 kb Pmp22/reporter construct is weakly developmentally regulated 3-4 The -8.5 kb Pmp22/reporter construct s not reactivated du ring nerve regeneration 3-5 The -8.5 kb Pmp22/reporter construct preferentially utilizes transcription fram exon lb Chapter 4: The expression of -21 kb promoter construct 4-1 Production and Characterization of transgenic mice 4-2 Different patterns of expression 4-3 Targeting to Schwann cells 4-4 Developmental regulation 4-5 Regeneration 4-6 Utilization of lA vs. lB promoters 4-7 Expression at the hprt locus Chapter 5: The expression of 100 kb gene construct 5-1 Production and Characterization oftransgenic mice 5 5-2 The 100 kb Pmp22/LacZ transgene targets expression to Schwann ceUs in aduZt mice.5-3 Prenatal expression of the 100 kb Pmp22/LacZ transgene 5-4 Developmental regulation of the 100 kb Pmp22/LacZ transgene 5-5 Expression of of the 100 kb Pmp22/LacZ transgene during peripheral nerve regeneration.5-6 Promoter utilization Chapter 6: Discussion Conclusion and future perspectives Appendix 1 Sequence comparisons Appendix 2 Animal use compUance certificate Appendix 3 Radioactive material use compliance certificate References 1 am grateful to my supervisor, and mentor Dr. G J Snipes
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".