First Report of Cactodera estonica in Canada
Bibliographic record
Abstract
A population of cyst nematode with terminal protuberance was found in soil samples associated with and on the roots of Polygonum aviculare L. at N45°23.480′, W75°40.463′ in South Ottawa, Ontario. Both J2 and cysts were found. It was subsequently confirmed with morphological and molecular methods as Cactodera estonica Krall and Krall, 1978 (Krall and Krall, 1978). The cysts are dark brown, elongated with length/breadth ratio more than 2, and vulval fenestra circumfenestrate 33 ± 4.1 (20–48) µm in diameter. Vulval slit is short 16 ± 2.7 (13–20) µm. Not all cysts have bullae or underbridge, when present a few bullae scattered on the ventral side of the cyst wall and the underbridge is thin; vulval denticles were found in one specimen. Male not found. J2 measurements are body length at 505 ± 45 (420–630) µm, stylet 25 ± 4.4 (22–29) µm long, and tail 36 ± 3.1 (34–38) µm with a short hyaline portion 14 ± 3.0 (12–19) µm. Lateral fields with four incisures. Tail end is round. All of these are consistent with those of C. estonica, for which the elongated cyst and short hyaline in J2 are characteristic for the species. Ribosomal DNA of the ITS, 18S, and D2/D3 of 28S regions were PCR amplified from cysts and J2s using primers 18S (5′-TTGATTACGTCCCTGCCCTTT-3′) and 26S (5′-TTTCACTCGCCGTTACTAAGG-3′) (Vrain et al., 1992), D2A (5′-ACAAGTACCGTGAGGGAAAGT-3′) (Nunn, 1992) and D3B (5′-GACCCGTCTTGAAACACGGA-3′) (De Ley et al., 1999), and sequenced. The sequences of the ITS and D2/D3 regions of 1,480 and 1,363 bps, respectively, were deposited in Genbank with accession numbers MF774482 and MF774483. When blasted in to the NCBI database (August 2017), the ITS sequence was 99% similar to sequences of AF274417, a population of C. estonica from Belgium and of KX579922 from Turkey, and the D2/D3 sequence was 99% similar to sequences of HM560797 and HM560796, populations of C. estonica from China. This is the first reported case of this nematode species in Canada and the second for the western hemisphere (Norgren and Golden, 1986).
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".