Comparative genetic analysis of Crocanthemum canadense (L.) Michx. plants of known pedigree (Chasmogamous- or cleistogamous-derived seeds) using amplified fragment length polymorphisms.
Bibliographic record
Abstract
Crocanthemum canadense(L.) Michx., commonly known as Rockrose, is an endangered plant native to northeastern North America. Rockrose has a dual breeding system producing two types of flowers in different locations on the same plant in the same growing season: chasmogamous (cross-fertilizing) flowers and cleistogamous (self-fertilizing) flowers. Yorke et al. (2011) showed Nova Scotia populations of Rockrose had the lowest genetic variation of those examined in its northeastern limit. These results left the question of whether an underlying reason exists beyond disjunction, such as reproductive influences, for the reduced genetic variation in Nova Scotia. Additionally, several new populations were discovered in Annapolis Valley along Highway 201 and on the Greenwood Air Force Base. Amplified fragment length polymorphisms (AFLPs) were used to measure and compare genetic diversity of plants of known pedigree and individuals belonging to newly discovered populations. Data were examined with analysis of molecular variance (AMOVA), principal coordinate analysis (PCoA), and a neighbour-joining tree to illustrate relationships among individuals and populations. It was determined no significant genetic variance existed between plants raised from cleistogamous or chasmogamous seeds. Very little genetic diversity was found between new populations examined, and a comparison against old populations also revealed very little genetic variation. Low population variance is likely due to continual inbreeding and low rates of cross-fertilization due to isolated, small populationspresent in Nova Scotia. Implications from this research may inform future conservation decisions. Potential breeding projects can use seeds produced by either cleistogamous or chasmogamous flowers without compromising the genetic integrity of Nova Scotia populations.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".