Supporting Information: The Substrate-Bound Crystal Structure of a Baeyer−Villiger Monooxygenase Exhibits a Criegee-like
Bibliographic record
Abstract
Subcloning the chnB1 gene into the pJW234 expression vector The chnB1 gene was amplified using Pwo DNA polymerase (Roche) from the pSDRmchnB1 plasmid. 1 PCR primers with NsiI and EcoRI restriction sites were used to amplify the gene (restriction sites are underlined), as shown in Table S2. The desired band was purified using the QIAquick Gel Extraction Kit (Qiagen) and subcloned into the pPCR-Script Cam vector (Stratagene) using the PCR-Script Cam Cloning Kit (Stratagene). Plasmid DNA from positive clones was prepared using the QIAprep Spin MiniPrep kit (Qiagen), and then digested using NsiI and EcoRI (New England BioLabs). The pJW234 expression vector (obtained from Dr. Mirek Cygler, University Saskatchewan, Saskatoon, Canada) was digested with the same enzymes. The desired bands from both digests were purified using gel extraction (MO BIO Laboratories UltraClean 15 DNA Purification Kit). The chnB1 insert was ligated into the pJW234 vector, and this plasmid was used to transform E. coli DH5α cells. Plasmid DNA from positive clones was prepared using the QIAprep Spin MiniPrep kit (Qiagen), and DNA sequencing was used to confirm the sequence (McGill University and Genome Quebec Innovation Centre Sequencing Service, Montreal, Canada). The resultant plasmid is called His8-TEV-ChnB1.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.003 |
| Meta-epidemiology (narrow) | 0.003 | 0.001 |
| Meta-epidemiology (broad) | 0.005 | 0.001 |
| Bibliometrics | 0.001 | 0.005 |
| Science and technology studies | 0.002 | 0.000 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.006 | 0.001 |
| Research integrity | 0.002 | 0.003 |
| Insufficient payload (model declined to judge) | 0.382 | 0.061 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".