DOI: 10.1126/science.1124513Supporting Online Material Materials and Methods Cloning
Bibliographic record
Abstract
Based on H3 numbering (S1), cDNA corresponding to residues 11–329 (HA1) and 1–176 (HA2) of the ectodomain of the hemagglutinin (HA) from A/Vietnam/1203/2004 (Viet04; Accession No. AY651334 & AY818135) (S2), A/Duck Alberta/35/1976 (H1_Duck; Accession No. AF091309 & D10477) and A/Duck/Singapore/Q-F119-3/1997 (H5-Duck; Accession No. AF303057) was cloned into the baculovirus transfer vector, pAcGP67-A (BD Biosciences Pharmingen, San Jose, CA). In a similar fashion to the construct used for expression of the 1918 influenza virus HA for structural studies (S3), the construct also incorporated a C-terminal thrombin cleavage site, a "foldon " sequence (S4) and a hexa His-tag at the extreme C terminus of the construct to enable protein purification. Once sequence verified, the plasmid was used for mutation studies. Primers containing point mutations around the receptor-binding site (see Figure 1) were designed using the web based program, PrimerX
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.003 | 0.002 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.002 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.005 | 0.003 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.003 | 0.005 |
| Insufficient payload (model declined to judge) | 0.405 | 0.420 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; the direct Gemma label and the distilled Codex classifier agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".