1 Cell Host & Microbe, Volume 2 Supplemental Data Heparan Sulfate Proteoglycans Provide a Signal to Plasmodium Sporozoites to Stop Migrating and Productively Invade Host Cells
Bibliographic record
Abstract
Isolation of mouse dermal fibroblasts (MDFs). 1 cm x 1 cm strips of skin from the back of a male Balb/C mouse were soaked in penicillin/streptomycin for 3 min before mincing into 1 mm x 1 mm pieces under sterile conditions. Skin pieces were then incubated in Liberase III (Roche Applied Sciences) for 1 hr at 37°C with agitation followed by grinding with PBS/0.2 % BSA and centrifugation for 10 min at 100 x g. The tissue was then filtered through 70 µm mesh, centrifuged, resuspended in DMEM/FCS and transferred to a 25-cm2 culture flask. Disaccharide Analysis. Cell lysates were treated overnight with 2 mg/ml Pronase followed by purification of the glycopeptides by anion exchange chromatography using DEAE Sephacel. Columns were washed with 0.25 M NaCl in 20 mM sodium acetate, pH 6.0 and eluted with 2 M NaCl. Glycans were released from peptides by β-elimination and repurified by anion exchange chromatography. Uronic acid concentration was determined by carbazole assay (Bitter and Muir, 1962) and HS composition was determined as follows: Chains were digested with heparin lyases I, II, and III (Ibex, Montreal, Canada), and the
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.380 | 0.199 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; the direct Gemma label and the distilled Codex classifier agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".