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Record W7099225715

SUPPORTING ONLINE MATERIAL

2016· article· en· W7099225715 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

aboutThe title or abstract carries a Canadian signal from the geographic lexicon.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

Venuenot available
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicInsect Utilization and Effects
Canadian institutionsnot available
Fundersnot available
KeywordsRiboprobeGenBankLarvaComplementary DNAInstarIn situ hybridization
DOInot available

Abstract

fetched live from OpenAlex

Florida, USA. Colonies were maintained in plastic boxes with glass test tubes filled with water constrained by cotton wool, and were fed a combination of mealworms, cockroaches, crickets, sunflower seed, and Bhatkar-Whitcomb diet (31). All colonies were maintained at 27 °C, 70 % humidity, and 12 hour day:night cycle. Isolation of sal homologues. We isolated fragments of sal from P. morrisi (1), as well as from P. obtusospinosa and P. rhea using PCR on cDNA that was generated from reverse transcription of their larval mRNA. We cloned and sequenced these fragments to confirm their identity, and performed gene tree analyses to confirm their orthology with Nasonia, Apis, and Drosophila sal. PCR primers and conditions are described in (1). GenBank accession numbers are JN205075 for P. obtusospinosa sal and JN205076 for P. rhea sal. Whole mount in situ hybridization and immunohistochemistry. We fixed and processed last instar larvae just prior to the prepupal stage (32). We carefully dissected larvae under a Zeiss Discovery V12 stereomicroscope to remove obstructive tissues surrounding imaginal discs. We then synthesized a digoxigenin-labelled riboprobe (Roche Diagnostics Canada) for P. morrisi sal (33). We used this P. morrisi sal probe for all in situ

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.436
Threshold uncertainty score0.989

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0110.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.251
Teacher spread0.233 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Quick stats

Citations0
Published2016
Admission routes1
Has abstractyes

Explore more

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