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Rapid detection of fibrinolytic activation in postpartum hemorrhage and acute obstetric coagulopathy using a novel assay

2025· article· en· W7117161304 on OpenAlexaff
Anton Ilich, Claire S. Whyte, Chatphatai Moonla, Callie Berkowitz, Izabela J. Pawlinski, Supreet Kaur Goraya, Ausra S. Lionikiene, P. Vincent Jenkins, L. de Lloyd, Sarah Bell, Rachel Collis, P. W. Collins, Nicola J. Mutch, Nigel S. Key

Bibliographic record

VenueBlood Advances · 2025
Typearticle
Languageen
FieldMedicine
TopicTrauma, Hemostasis, Coagulopathy, Resuscitation
Canadian institutionsInstitute of Infection and Immunity
FundersMedical Research ScotlandFriends of ANCHORBritish Heart FoundationNational Institute of Academic Anaesthesia
KeywordsFibrinolysisCoagulopathyPlacental abruptionAmniotic fluid embolismPlasminHemostasisCoagulationPregnancy

Abstract

fetched live from OpenAlex

ABSTRACT: Postpartum hemorrhage (PPH) remains the leading cause of pregnancy-related mortality worldwide. Regardless of the initiating cause, continued bleeding may progress to a systemic coagulopathy. This coagulopathy may be complicated further by profound fibrinolytic activation that progresses to systemic hyperfibrinolysis, a condition that we have termed acute obstetric coagulopathy (AOC). Patients with placental abruption or amniotic fluid embolism are among those at highest risk for AOC. In response to the unmet need for a rapid method to detect fibrinolytic activation in this scenario, we developed a novel assay that we have termed the fibrinolytic activity screening test (FAST). This assay measures endogenously generated plasmin activity in plasma within 5 minutes. Its high sensitivity for the detection of in vivo fibrinolytic activation was confirmed by strong correlation with elevated plasmin-antiplasmin (PAP) complex levels. We analyzed archived plasma samples from 33 women with PPH and 20 pregnant women just before elective cesarean delivery. Of the 33 patients with PPH, 12 had PAP complex levels >25 000 ng/mL, thereby meeting criteria for the diagnosis of AOC. Plasmin activity measured by the FAST assay differentiated AOC from non-AOC PPH (P = .0007) and from pregnant non-PPH control groups (P< .0001) and was strongly correlated with both PAP complex and D-dimer levels. Among patients with PPH for whom viscoelastic whole blood testing (ROTEM) was performed, none of the 18 without AOC or 9 of those with AOC had evidence of ROTEM-defined hyperfibrinolysis. The FAST assay is a rapid tool to detect activation of fibrinolysis associated with AOC in women after delivery.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.182
Threshold uncertainty score0.699

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.283
Teacher spread0.265 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2025
Admission routes1
Has abstractyes

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