Zinc-Finger 5 Is an Activation Domain in the Saccharomyces cerevisiae Stress-Responsive Transcription Factor Fzf1
Bibliographic record
Abstract
Fzf1 is a Saccharomyces cerevisiae transcription factor that contains five zinc finger domains (ZF1-5) and induces the expression of at least five genes in response to various chemical stresses by recognizing the shared promoter consensus sequence CS2. The N-terminal ZF1-3 are required and sufficient for binding to CS2, while ZF4 negatively regulates the activity of Fzf1. However, the effect of ZF5 on the activity of Fzf1 is not well defined. In this study, substitutions of the two zinc-coordinating Cys residues (C248S and C253S) of ZF5, or deletion of the whole ZF5 domain, compromised the chemical stress-induced activation of Fzf1. Since the elevated Fzf1-regulated gene expression caused by fzf1-ZF4 could also be reversed by additional deletion of ZF5 or C248S/C253S substitutions, fzf1-ZF5 mutations are epistatic over fzf1-ZF4 mutations. Furthermore, fzf1-ZF5 mutations are recessive to FZF1, while ZF5 is dispensable for the CS2 binding. Finally, Fzf1-ZF5 is required and sufficient to serve as a transcription activation domain when fused to a Gal4 DNA-binding domain. These observations collectively support a working model in which Fzf1 bound to its target gene promoters remains inactive due to an inhibitory activity of ZF4. Upon chemical stress, ZF4 is no longer able to inhibit the ZF5 transactivation activity, leading to the induction of Fzf1-regulated gene expression and subsequent chemical detoxification.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".