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Record W7123773215 · doi:10.36939/ir.202601131603

Environmental DNA as a tool to supplement sampling for fish community monitoring

2025· dissertation· en· W7123773215 on OpenAlexaboutno aff
Andrew Klein

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldEnvironmental Science
TopicEnvironmental DNA in Biodiversity Studies
Canadian institutionsnot available
Fundersnot available
KeywordsEnvironmental DNASampling (signal processing)Catch per unit effortBiomass (ecology)Fish <Actinopterygii>Spring (device)

Abstract

fetched live from OpenAlex

Environmental DNA (eDNA), the genetic material shed by organisms into the environment, is a promising tool for fisheries monitoring due to its non-lethal, and efficient sampling. My thesis evaluates the use of eDNA as a complementary approach to gillnet surveys within the Coordinated Aquatic Monitoring Program (CAMP) in Manitoba. The primary objectives of my thesis were to assess (1) whether seasonal variation and site differences influence eDNA detectability of target species, and (2) whether eDNA concentration is associated with traditional measures of relative abundance, including Catch Per Unit Effort (CPUE) and Biomass Per Unit Effort (BPUE). Water samples were collected at various time points from Lac du Bonnet and Pointe du Bois, Winnipeg River system, and analyzed with species-specific assays for five fishes: Walleye (Sander vitreus), Spottail Shiner (Hudsonius hudsonius), Yellow Perch, (Perca flavescens), Trout-perch (Percopsis omiscomaycus), and Burbot (Lota lota). Seasonal eDNA sampling detected all species on both waterbodies during spring and fall, except for Burbot, which was not detected at one of the three sites in the fall. eDNA and gillnetting results showed consistent detections for four of the five species, with Burbot detected more often by eDNA than gillnets. While most species showed weak association between eDNA and CPUE/BPUE, Burbot displayed a significant positive relationship. These findings demonstrate that eDNA has the potential to expand detection of underrepresented species and enhance monitoring programs.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.004
metaresearch head score (Gemma)0.006
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.015
Threshold uncertainty score0.029

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0040.006
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0040.003
Science and technology studies0.0010.001
Scholarly communication0.0010.001
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.032
GPT teacher head0.281
Teacher spread0.249 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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