Isolation, Characterization, and Feed Application of a Halotolerant Phytase-Producing Streptomyces sp. from Indonesian Mangrove Sediments
Bibliographic record
Abstract
Phytase is a key enzyme for improving phosphorus availability in poultry feed by hydrolyzing phytic acid.This study screened actinobacteria isolated from mangrove litter in Segara Anakan, Cilacap, for phytase production and evaluated their application in poultry feed ingredients.Twelve actinobacterial isolates exhibited phytase activity, with isolate K-2C showing the highest activity (3.42 U mL under optimal conditions).Molecular identification based on the 16S rRNA gene revealed 99.02% similarity of K-2C to Streptomyces griseorubens NBRC 12780.Maximum phytase production was achieved after two days of incubation, with optimal activity at pH 7.0 and 50.In vitro application of K-2C phytase to rice husk, corn flour, soybean meal, and pollard significantly increased inorganic phosphorus release after 12 h of incubation, with phosphorus levels rising by 28.6-61.3%compared to controls.The highest phosphorus release was observed in rice husk, while corn flour showed the lowest response.These findings demonstrate that actinobacteria from mangrove litter are a promising indigenous source of phytase for reducing phytic acid and enhancing phosphorus availability in poultry feed.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".