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Record W7132622264

In vitro propagation of Vitis

2016· article· en· W7132622264 on OpenAlexaboutno aff

Bibliographic record

VenueFederal Research Centre for Cultivated Plants (Julius Kühn-Institut) · 2016
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicHorticultural and Viticultural Research
Canadian institutionsnot available
Fundersnot available
KeywordsCallusProtoplastSomatic embryogenesisCultivarPlant developmentOrganogenesis
DOInot available

Abstract

fetched live from OpenAlex

a-vitro-Vermehrung von VitisZusammenfassung.-Es wird eine in-vitro-Methode beschrieben, mit deren Hilfe in 4 Monaten aus einem einzigen Sprostckchen von 3-5 mm Lnge ber 12.000 Triebe gezogen werden knnen.Bei der franzsischen Rebkreuzung Baco, die auf organischem Minimalnhrboden nach Murasbige (MMO) mit Zustzen von 80 mg Adeninsulfat/l, 170 mg einbasigem Natriumphosphat/! und 3-4 mg N6-enzylaminopurin/l (BAP) kultiviert wurde, war die Sproproduktion auf das 7fache gesteigert, wenn zuerst eine 4wchige Anzucht auf festem Nhrboden (0,7 g Agar/l) und danach eine bertragung in flssiges Nhrmedium erfolgte.Auf dem gleichen Substrat wurden weitere 21 Rebsorten vermehrt, wobei die BAP-Menge auf 2 mg/l und das bertragungsintervall von 3 auf 2 Wochen herabgesetzt waren.Ober 90 % der Triebe bewurzelten sich im Verlauf von 1-2 Wochen ohne Schwierigkeiten, wenn sie auf Filterpapierbrcken gebracht wurden, die in flssigem, auf 1/4 verdnntem MMO standen, das mit 150 mg einbasigem Natriumphosphat/!, 10 g Saccharose/!und 0,1 mg Indolyl-3essigsure/l angereichert war; innerhalb von 2-3 Wochen bewurzelten sie sich auch in grobem Perlit, das mit denselben Nhrstoffen, aber ohne Saccharose, gesttigt war.lntroduction Canadian grape growers and wineries are constantly looking for new hardy grape cultivars suitable for producing superior wines.However, according to regulations, all vines planted must come from a certified virus-free source.Since only one plant is usually certified virus-free, it takes several years to propagate enough vines by conventional methods.On the other hand, the development of in vitro methods for many plant species provides a promising method for the rapid propagation of grapes.Previous work on callus and protoplast culture, embryogenesis and organogenesis in Vitis and virus eradication methods described by GIFFORD and HEWITT (1961), GALzy (1972) and BINI (1976) had Iittle significance for developing propagation methods.Later, however, JoNA and WEBB (1978) sucessfully propagated V. vinifera Sylvaner Riesling from lateral buds and BARLASS and SKENE (1978, 1980} propagated Cabernet Sauvignon from fragmented shoottips.The Saanichton Research and Plant Quarantine Station has been developing in vifro methods for virus eradication, rapid propagation, and Vitis germ-plasm storage.The propagation methods are described in the present paper. Materials and methodsApical shoot-tips from virus-free plants of the French hybrid cv.Baca growing in a greenhouse during the winter and a shadehouse during the summer were used to develop the procedure.The method was Iater tested on 21 V. vinifera and hybrid genotypes.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.002
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.080
Threshold uncertainty score0.550

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.002
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0010.000
Scholarly communication0.0000.001
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.062
GPT teacher head0.320
Teacher spread0.258 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2016
Admission routes1
Has abstractyes

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