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Record W7132875590

Transcriptional regulation of the murine CD45 gene

2004· dissertation· W7132875590 on OpenAlexaff
Un Kyong Kwon

Bibliographic record

VenueTSpace · 2004
Typedissertation
Language
FieldImmunology and Microbiology
TopicT-cell and B-cell Immunology
Canadian institutionsLibrary and Archives Canada
Fundersnot available
KeywordsTranscription (linguistics)Transcriptional regulationGeneTranscription factorMyeloidRegulation of gene expressionCell cultureGene isoform
DOInot available

Abstract

fetched live from OpenAlex

The mechanisms governing the lineage-specific transcriptional regulation of CD45 are unknown. CD45 utilizes three mutually exclusive promoters: P1a, P1b, and P2. It was found that transcription of CD45 primarily initiated from P1b in the myeloid and lymphoid lineages in all hematopoietic cell lines and primary cells tested, except for the thymoma cell line EL4. Real-time RT-PCR assays with a series of reporter constructs that covered various upstream sequences showed that an element between -438 and -420 upstream of P1b was identified to repress transcription from P1a in M12 (B lymphoma) and EL4 and activate transcription from P1b in RAW264.7 (Macrophage). EMSA identified that Oct-1 binds this region in RAW264.7, M12, and EL4. In addition, Oct-2 binds this region in EL4. Therefore, the Octamer factors are involved in the transcriptional regulation of CD45 in both the myeloid and lymphoid lineages.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.258
Teacher spread0.245 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2004
Admission routes1
Has abstractyes

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