Molecular mechanism of protein kinase C enhancement of N-methyl-D-aspartate receptor calcium-dependent inactivation
Bibliographic record
Abstract
Glutamate is the major excitatory neurotransmitter in the central nervous system. N-Methyl-D-aspartame receptors (NMDAR) belong to the family of ionotropic glutamate receptors, implicated in synaptic plasticity, synaptogenesis, excitotoxicity, and the induction of long term potentiation. The NMDA receptor subunit, NR1, has a large intracellular C-terminal tail that has been shown to bind both Calmodulin (CaM) and α-actinin, and has been shown to be a target of PKC and PKA phosphorylation. We proposed that PKC phosphorylation of certain serine residues on the NR1 subunit can enhance calcium-dependent inactivation of the NMDAR through the rearrangement of Calmodulin and α-actinin binding. Through the use of whole-cell recording of recombinant receptors expressed in HEK293 cells, site-directed mutagenesis of the NR1 subunit, and immuno-precipitation combined with Western blotting techniques, we conclude that the NR1 C-terminus is not involved in the PKC enhancement of calcium dependent inactivation in HEK293 cells expressing NR1-1a/NR2A subunits.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".