Characterization of Abscisic Acid Transport in Arabidopsis thaliana and Brassica napus
Bibliographic record
Abstract
The phytohormone abscisic acid (ABA) plays an important role in regulating numerous plant processes such as seed maturation, seed dormancy, and stress responses. These regulations require the long-distance movement of ABA in plants, which has been documented and requires a proper network of ABA transporters. Positron Emission Tomography (PET) in planta allows a real-time and non-destructive method for studying the movement of small molecules using a radiotracer. We show that 3’-F-ABA displayed similar bioactivity as ABA via ABA-responsive β-glucuronidase (GUS) reporter expression in Arabidopsis thaliana. In addition, the destructive phytochemical analysis of Brassica napus plants co-applied 3’-F-ABA and deuterium-labeled ABA (d6-ABA) showed a similar distribution pattern. Arabidopsis transporter mutants (abcg25, abcg40, ait1) showed delayed GUS expression in shoots which suggests that ABCG25, ABCG40, and AIT1 are required for the quick transport of ABA from root to shoot. Lastly, several ABA analogs were found to be candidates for ABA transport inhibitors.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".