Mechanisms of locomotor recovery after spinal cord repair with peripheral nerves, fibroblast growth factor 1, and fibrin glue after complete spinal cord transection in the adult mammal
Bibliographic record
Abstract
A simultaneous, bi-directional axonal tracing technique was developed using anterograde DiI and retrograde Fluoro-Gold that was compatible with immunohistochemistry. With the use of this technique, it was determined that functional recovery after CSCT and repair correlated with regeneration of corticospinal tract axons into the grey matter of the lumbar spinal cord. Intentional circumvention of white matter inhibition was not found to be necessary for functional recovery. In conclusion, recovery of function with peripheral nerves, FGF1, and fibrin glue resulted in recovery of function which was associated with regeneration of corticospinal tract axons into the grey matter of the lumbar spinal cord. The success of the functional recovery after repair was not dependent on deliberate circumvention of white matter inhibition. Further study is required to determine other factors that may improve or abolish functional recovery after repair. Although repair after spinal cord transection in the adult mammal had been believed unattainable, in the last decade several reports have documented regeneration of axons and recovery of function after complete spinal cord transection (CSCT) and repair. While both axonal regeneration and functional recovery have been shown after repair, it is unclear whether recovery is due to the axonal regeneration and, if so, which axons are involved. The purpose of this study was to determine which axonal tracts were involved in recovery of function after repair with peripheral nerve grafts, fibroblast growth factor 1 (FGF1), and fibrin glue, and to determine the effect of white matter inhibition on functional recovery. All experiments were performed on adult rats in accordance with policies established by the Canadian Council of Animal Care. The first series of experiments developed the optimal methods for axonal tracing and immunohistochemistry to study axonal regeneration in the spinal cord after CSCT and repair. The second series of experiments determined which axons were correlated with function after CSCT and repair, and whether intentional circumvention of white matter inhibition was necessary for functional recovery.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".