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Record W7133027463

Uncovering the structural basis for the mechanical properties of elastin

2025· dissertation· W7133027463 on OpenAlexaff
Elnaz Mirhaji

Bibliographic record

VenueTSpace · 2025
Typedissertation
Language
FieldBiochemistry, Genetics and Molecular Biology
TopicConnective tissue disorders research
Canadian institutionsUniversity of Toronto
Fundersnot available
KeywordsPeptideMolecular dynamicsElastinStiffnessHydrophobic effectCopolymerElastic recoilDomain (mathematical analysis)
DOInot available

Abstract

fetched live from OpenAlex

The mechanical properties of elastin, including its elasticity, resilience, and stress relaxation, arise from its unique block copolymer structure, comprising hydrophobic (HD) and cross-linking (CLD) domains. To investigate the role of molecular structures of individual hydrophobic and cross-linking domains in modulating mechanical properties, we performed extensive atomistic molecular dynamics simulations of three peptides: hydrophobic [h = (GVPGV)7], cross-linking (x = A5KAAKYGA) domains, and a hybrid peptide (h-x = GVPGVA5KAAKYGA). The study of the h-x peptide allowed us to examine the interplay between hydrophobic and cross-linking domains and their combined effect on structural and mechanical properties. At equilibrium, all peptides sampled heterogeneous and highly disordered structural ensembles. Hydrophobic peptide h predominantly adopts sparse and transient β-turns, while peptides x and h-x form transient α- and 310-helical structures with moderate propensities. Distinct mechanical properties were uncovered for the single domain h and both cross-linking peptides. Peptide h showed high extensibility, low elastic modulus, and elastic recoil primarily driven by the hydrophobic effect, whereas peptides x and h-x displayed greater stiffness and low extensibility consistent with the structural roles of HDs and CLDs in elastin. Peptide h-x shows a biphasic response to strain, with changes in structural properties similar to those of hydrophobic domain h at lower strains and to those of cross-linking domain x at higher strains underlying the decoupling of mechanical properties of h and x domains. Together, these findings support a simple elastic network model attributing elastin's extensibility and elastic recoil to the properties of HDs, while CLDs contribute to its structural integrity. The single domain h undergoes significant stress relaxation characterized by structural rearrangements toward a more homogeneous and disordered conformational ensemble. This relaxation from its extended state is predominantly driven by conformational entropy. Ultimately, this knowledge will help in understanding elastin's remarkable mechanical properties.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.032
GPT teacher head0.350
Teacher spread0.318 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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