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Record W7133070792

Targeting the TIGIT/CD226/CD96 Signaling Axis using the IgV Domain of PVR

2023· dissertation· W7133070792 on OpenAlexaff
Shrayasee Saha

Bibliographic record

VenueTSpace · 2023
Typedissertation
Language
FieldImmunology and Microbiology
TopicImmune Cell Function and Interaction
Canadian institutionsUniversity of Toronto
Fundersnot available
KeywordsReceptorImmune systemSignal transductionExtracellularTIGITAutoimmune diseaseDomain (mathematical analysis)Recombinant DNA
DOInot available

Abstract

fetched live from OpenAlex

PVR (poliovirus receptor) functions as a ligand that signals through TIGIT and CD96 to induce suppression of T-cell and NK-cell responses and binds to CD226, resulting in a co-stimulatory signal. PVR and its immune-cell receptors have been implicated in several diseases including cancer and autoimmune disorders. Therefore, my thesis focused on engineering a soluble version of the PVR extracellular domain that could serve to modulate immune responses responsible for such diseases. We developed vdPVR-Fc by fusing the murine IgV binding region of PVR to the Fc-domain of human IgG1. vdPVR-Fc bound to recombinant forms of its receptors TIGIT, CD96 and CD226 with similar low nanomolar affinities. Additionally, vdPVR-Fc could suppress murine T-cell activity ex-vivo. Importantly, vdPVR-Fc suppressed inflammatory responses in-vivo by reducing disease severity in murine models of psoriasis and colitis. Collectively, these results demonstrate that the minimal IgV domain of PVR is sufficient to dampen T-cell mediated immune responses.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.026
GPT teacher head0.314
Teacher spread0.288 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

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