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Record W7161851149 · doi:10.82308/21596

Case studies in vaccine evaluation

2018· dissertation· en· W7161851149 on OpenAlexaboutno aff
Janna Shapiro

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicTransgenic Plants and Applications
Canadian institutionsnot available
Fundersnot available
KeywordsImmune systemVaccinationPeripheral blood mononuclear cellVaccine efficacyClinical trialCytokineAntibodyInfluenza vaccine

Abstract

fetched live from OpenAlex

Vaccines are estimated to prevent 2.5 million deaths each year. As with any public health intervention, vaccines progress through many stages of development and their evaluation must continue post-licensure. We have used case studies to highlight four key aspects of the lifecycle of vaccines: i) preclinical evaluation, ii) clinical development, iii) safety and iv) long-term effectiveness. Preclinical evaluation - We have demonstrated, for the first time, cellular immune responses to a candidate norovirus virus-like particle vaccine produced in Nicotiana benthamiana (Medicago Inc., Ste-Foy, QC). Both oral and intramuscular formulations of the vaccine elicited a proliferative response and IFNγ-producing cells in mice, and antibody-secreting cells in both mice and rabbits. Clinical development - In collaboration with MIT Canada Inc., an 80-subject clinical trial was performed to evaluate the use of the MedJet jet injection device to deliver seasonal influenza vaccines. Both cellular and humoral immune responses to a trivalent vaccine delivered by the MedJet were equivalent to those delivered by needle-and-syringe. Of particular interest, there were no significant differences in the frequency of responding memory CD4+ T cells after re-stimulation or in the degree of poly-functionality of these cells in the healthy adult subjects. Vaccine safety – We performed a detailed immunologic investigation of peripheral nerve demyelination in a young woman that occurred in temporal association with human papillomavirus vaccination (Gardasil™). Working with peripheral blood mononuclear cells from the patient in vitro, unusually strong proliferative and cytokine responses were found to vaccine antigens, primarily driven by B cells. These data support, but do not prove, an association between the vaccine and peripheral nerve paralysis. Long-term effectiveness - To monitor the long-term persistence of antibodies after the rubella vaccination, we developed, optimized and validated a low-cost, serum-sparing ELISA to measure serum anti-rubella IgG titers. In collaboration with the Public Health Agency of Canada, this assay was used to assess rubella immunity in 1,752 pregnant women across Canada. This study (Vaccine 2017) highlighted socio-demographic inequalities in vaccine uptake and the need to address these issues in underserved populations. Together, these studies illustrate that the life-saving potential of vaccines relies on continuous evaluation of their safety and effectiveness throughout the vaccine lifecycle; both pre- and post- licensure.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.111
Threshold uncertainty score0.490

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.054
GPT teacher head0.397
Teacher spread0.343 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2018
Admission routes1
Has abstractyes

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